Flow Cytometry Staining and Analysis: Univariate Histograms

Flow Cytometry Staining and Analysis: Univariate Histograms

NG6 cells (Clone of NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells) were stained with 4 μg of mup75 antibody for 1 hr, washed, and subsequently incubated for 30 minutes with Anti-Rabbit FITC (2 μg). Data acquired on a BD FACScan and processed with CellQuest software. 91% positive shift seen.