Fluorescent Microscopy – Cytologistics

The application of an array of fluorochromes makes it possible to identify cells and cellular components with a high degree of specificity amidst non-fluorescing material. A fluorescence microscope is capable of revealing the presence of a single molecule, and through the use of multiple labeling, different probes can identify several target molecules simultaneously. The fluorescence microscope has the capability of detection of fluorescing molecules below the diffraction limit of specific specimen features.

Immunocytochemistry — HEK-293 cells were transfected with the p75 neurotrophin receptor and were labeled with Cy3 labeled 192-IgG (20 ug/ul). Analysis performed on a Leica DMIL Fluorescent Microscope. Image was captured at 20X magnification using SPOT imaging software.