Cell Sorting Questionnaire

Samples should be at a minimum concentration of 5 x 10 to the 6 cells/ml.

Also please be aware of the following:

  • Resuspend and deliver your cells in PBS + 2% FBS
  • If you are aware that your cells have the tendency to clump, please filter your samples for aggregates or debris with a 40-45 micron mesh filter. Keep in mind that filtration of your cells will tend to decrease your cell number, so make sure you begin with adequate cell numbers. 50-µm Nitex Nylon Mesh (Genesee Scientific Cat #57-106) or 40-µm cell strainer cap (BD Falcon Cat #352340). Keeping cells suspended in 1-5% BSA/media or EDTA (0.02%) can also help.
  • Provide samples in 5 ml Falcon 12 x 75 polystyrene tubes (BD Labware Cat # 352054).
  • Provide the appropriate culture media for collection of your sorted cells.
  • If your schedule will delay your arrival for more than 15 minutes, please give us a call.

Thank you for your attention to this information. We appreciate your business and look forward to working with you.