Also please be aware of the following:
- Resuspend and deliver your cells in PBS + 2% FBS
- If you are aware that your cells have the tendency to clump, please filter your samples for aggregates or debris with a 40-45 micron mesh filter. Keep in mind that filtration of your cells will tend to decrease your cell number, so make sure you begin with adequate cell numbers. 50-µm Nitex Nylon Mesh (Genesee Scientific Cat #57-106) or 40-µm cell strainer cap (BD Falcon Cat #352340). Keeping cells suspended in 1-5% BSA/media or EDTA (0.02%) can also help.
- Provide samples in 5 ml Falcon 12 x 75 polystyrene tubes (BD Labware Cat # 352054).
- Provide the appropriate culture media for collection of your sorted cells.
- If your schedule will delay your arrival for more than 15 minutes, please give us a call.
Thank you for your attention to this information. We appreciate your business and look forward to working with you.