Transfected cells are widely accepted for in vitro research and therapeutic applications. Use of antibiotics in the transfection process has several shortcomings such as cost, continuous use, slow-acting and harmful effects – even on the transfected cell population. In addition, selection pressures are often inefficient and fail to provide a population of cells that express the gene of interest (GOI) at high levels. To validate our new transfection technology, we used three separate GOI’s to select for solely high-expressing transfectants using targeted toxin selection pressure. This method of selection provides a means of purifying the highest expressing transfected populations using a more cost-effective and time-saving approach.
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