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Immunocytochemistry

Immunocytochemistry

HEK-293 cells were transfected with the p75 neurotrophin receptor and were labeled with Cy3 labeled 192-IgG (20 ug/ul). Analysis performed on a Leica DMIL Fluorescent Microscope. Image was captured at 20X magnification using SPOT imaging software.

Cytotoxicity Assay

Cytotoxicity Assay

Three Human Non-Small Cell Lung Cancer cells line were challenged with an antibody directed to a cell surface receptor conjugated to the ribosome inactivating protein saporin. Saporin alone was used as a control. This type of assay allos for a large number of targeting agents to be screened quickly and cost-efficiently for specificity, functional binding, […]

Flow Cytometry Staining and Analysis: Univariate Histograms

Flow Cytometry Staining and Analysis: Univariate Histograms

NG6 cells (Clone of NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells) were stained with 4 μg of mup75 antibody for 1 hr, washed, and subsequently incubated for 30 minutes with Anti-Rabbit FITC (2 μg). Data acquired on a BD FACScan and processed with CellQuest software. 91% positive shift seen.